In transgenic biotechnology involving bacteria, which sequence of events would be followed in the process of harvesting a protein of interest?
a. Replicate host bacterial cells; isolate gene that codes for protein; splice gene into plasmids; harvest protein of interest; transform plasmids into bacteria.
b. Transform plasmids into bacteria; isolate gene that codes for protein; replicate host bacterial cells; splice gene into plasmids; harvest protein of interest.
c. Harvest protein of interest; isolate gene that codes for protein; splice gene into plasmids; replicate host bacterial cells; transform plasmids into bacteria.
d. Isolate gene that codes for protein; splice gene into plasmids; replicate host bacterial cells; transform plasmids into bacteria; harvest protein of interest.
e. Isolate gene that codes for protein; splice gene into plasmids; transform plasmids into bacteria; replicate host bacterial cells; harvest protein of interest.