DNA is cut into useful fragments through use of the molecular scissors known as _______.

The plasmids noted in the text had ________ DNA spliced into them as a first step in using them to turn out a human _______.

One of the motives put forth for human cloning is that people want to replace children or other loved ones who have died. To what extent could a clone of a loved one be a replacement for that person? If the technique had then been available, should doctors in the nineteenth century have preserved the DNA of Abraham Lincoln for cloning?

How is biotechnology defined?

a. the study of technical biology

b. the use of biology to control technological processes as a means of meeting societal needs

c. the use of technology to control biological processes as a means of meeting societal needs

d. the technological process of cloning genes

e. the commercialization of biological research results

Should society demand that there be no risks to genetically modified foods before it allows them to be developed? Does any significant technology have no risks associated with it?

Researchers who work with DNA are able to cut it into desired lengths through the use of:

a. transformation.

b. cloning.

c. RNA.

d. restriction enzymes.

e. DNA polymerase.

What limits, if any, should be put on the ability of human beings to modify the genomes of other living things? Should human beings be free to carry out any modification that does not harm an organism?

In transgenic biotechnology involving bacteria, which sequence of events would be followed in the process of harvesting a protein of interest?

a. Replicate host bacterial cells; isolate gene that codes for protein; splice gene into plasmids; harvest protein of interest; transform plasmids into bacteria.

b. Transform plasmids into bacteria; isolate gene that codes for protein; replicate host bacterial cells; splice gene into plasmids; harvest protein of interest.

c. Harvest protein of interest; isolate gene that codes for protein; splice gene into plasmids; replicate host bacterial cells; transform plasmids into bacteria.

d. Isolate gene that codes for protein; splice gene into plasmids; replicate host bacterial cells; transform plasmids into bacteria; harvest protein of interest.

e. Isolate gene that codes for protein; splice gene into plasmids; transform plasmids into bacteria; replicate host bacterial cells; harvest protein of interest.

Which of the following is an example of a transgenic organism?

a. E. coli bacterium with a human gene inserted

b. Dolly the sheep

c. rice with bacterium and daffodil genes inserted

d. all of the above

e. a and c only

What is a clone?

a. an exact genetic copy of a gene or an entire living thing

b. a cell that can take up plasmids

c. a segment of DNA of a specified length

d. an offspring produced by test-tube fertilization

e. a segment of DNA that can prime the polymerase chain reaction

Dolly the sheep was cloned by means of:

a. applying an electric current to an egg and sperm in a laboratory.

b. taking an embryo from one sheep and implanting it in another.

c. taking a cell from an adult sheep and fusing it with a sheep egg that had its DNA removed.

d. inserting new genes into an existing mammary cell.

e. getting sperm to fuse with egg outside a living sheep.