Dolly was a clone of the sheep who ______.

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Answer:

Dolly was a clone of the sheep, which had no second parent, who had an exact copy of the DNA (deoxyribonucleic acid) of the donor sheep in its nucleus.

Dolly was the first mammal to be cloned from another adult mammal. Dolly was created through reproductive cloning technique. A cell was isolated from the udder of white sheep and allowed to multiply in vitro.

Simultaneously, an egg cell was isolated from a black-faced sheep. The nucleus of the egg cell was removed with the help of a micropipette. The cell, isolated from white sheep, which was allowed to multiply, divided into numerous daughter cells.

A single perfect udder cell was selected and was called ‘donor cell.’ The donor cell has its nucleus intact. The egg cell did not have nucleus, but had proteins, which were needed for embryonic development.

The donor cell and the egg cell were kept side by side and electric shock was applied to the egg cell. The shock triggered the fusion of egg cell with the donor cell. The DNA of the donor cell got reprogrammed and initiated the development of embryo. The egg cell already had all the proteins needed for embryo development. So, after a period of incubation, the embryo was implanted into a surrogate mother sheep.

Related Questions

A transgenic organism is one whose genome has stably incorporated one or more _______ from ________.

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DNA is cut into useful fragments through use of the molecular scissors known as _______.

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The plasmids noted in the text had ________ DNA spliced into them as a first step in using them to turn out a human _______.

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One of the motives put forth for human cloning is that people want to replace children or other loved ones who have died. To what extent could a clone of a loved one be a replacement for that person? If the technique had then been available, should doctors in the nineteenth century have preserved the DNA of Abraham Lincoln for cloning?

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How is biotechnology defined?

a. the study of technical biology

b. the use of biology to control technological processes as a means of meeting societal needs

c. the use of technology to control biological processes as a means of meeting societal needs

d. the technological process of cloning genes

e. the commercialization of biological research results

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Should society demand that there be no risks to genetically modified foods before it allows them to be developed? Does any significant technology have no risks associated with it?

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Researchers who work with DNA are able to cut it into desired lengths through the use of:

a. transformation.

b. cloning.

c. RNA.

d. restriction enzymes.

e. DNA polymerase.

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What limits, if any, should be put on the ability of human beings to modify the genomes of other living things? Should human beings be free to carry out any modification that does not harm an organism?

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In transgenic biotechnology involving bacteria, which sequence of events would be followed in the process of harvesting a protein of interest?

a. Replicate host bacterial cells; isolate gene that codes for protein; splice gene into plasmids; harvest protein of interest; transform plasmids into bacteria.

b. Transform plasmids into bacteria; isolate gene that codes for protein; replicate host bacterial cells; splice gene into plasmids; harvest protein of interest.

c. Harvest protein of interest; isolate gene that codes for protein; splice gene into plasmids; replicate host bacterial cells; transform plasmids into bacteria.

d. Isolate gene that codes for protein; splice gene into plasmids; replicate host bacterial cells; transform plasmids into bacteria; harvest protein of interest.

e. Isolate gene that codes for protein; splice gene into plasmids; transform plasmids into bacteria; replicate host bacterial cells; harvest protein of interest.

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Which of the following is an example of a transgenic organism?

a. E. coli bacterium with a human gene inserted

b. Dolly the sheep

c. rice with bacterium and daffodil genes inserted

d. all of the above

e. a and c only

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